Fig. 3.

Complex N-glycans as substrates for recombinant galactosidases. Dabsylated-GalGal glycopeptide derived from bovine fibrin was used as substrate for a A. niger rlacA and b A. nidulans rlacA. Additional enzyme (0.2 μg) was added every 24 h. An approximate amount of 5 % non-cleavable galactose, which corresponds to β-1,3-linked galactose remained in the A. niger rlacA reactions (a) and could be successfully removed upon the addition of bovine testes galactosidase at 96 h. The glycans are depicted following the glycan nomenclature of the Consortium for Functional Glycomics (http://www.functionalglycomics.org)