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. 2014 Mar 24;4(3):e113. doi: 10.1038/nutd.2014.10

Figure 3.

Figure 3

Effects of (a) FFA and LPS stimulation on MCP-1 mRNA expression and (b) MCP-1 protein secretion in 3T3-L1 adipocytes. 3T3-L1 adipocytes were incubated with DMEM growth media containing 2% FAF-BSA and 500 μM of BSA-complexed fatty acid for 24 h. The fatty acids investigated are: monounsaturated fatty acid (oleic acid/OA), saturated fatty acid (palmitic acid/PA) and trans fatty acid (elaidic acid/EA). Results were expressed as±s.e.m., relative to FAF-BSA=1 (n=9), ns=non-significant vs FAF-BSA.