Figure 2.

Effect of leptin on the migration of GC cells. (A) AGS and MKN-45 cells (1 × 10⁴ cells/well) were treated in the absence (control) or presence of various doses of leptin (10, 50, 100, and 500 ng ml⁻¹) for 24 h. The migration was measured using transwell assay. Scale bar, 50 μm. The migration numbers were expressed relatively to control (B). (C) In a separate experiment, AGS and MKN-45 cells were treated with leptin (100 ng ml⁻¹), and the migration was detected at 0, 6, 12, 24, and 36 h. All data shown were expressed as mean±s.d. of three independent experiments. The images were from a representative experiment. *P<0.05, **P<0.01.