Figure 4.

Leptin enhanced GC cell migration via upregulating ICAM-1. The expression level of ICAM-1 in GC cells was measured by WB after transfection with pcDNA3.1-ICAM-1 (A) or ICAM-1-siRNA (B). One representative result out of three independent experiments is shown. Graphs represent quantitation with β-actin as the internal standard. Each bar represents the mean±s.d. (n=3, **P<0.01). In addition, AGS and MKN-45 cells were transfected with pcDNA3.1-ICAM-1 (C) or ICAM-1-siRNA (D) for 24 h and then treated with leptin (100 ng ml⁻¹) for 24 h. The migration was measured by transwell assay. The migration numbers were expressed as relative to control. *P<0.05, **P<0.01.