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. 2004 Apr 1;101(15):5506–5511. doi: 10.1073/pnas.0306324101

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Copyright © 2004, The National Academy of Sciences

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Fig. 2. — Zap1p-dependent regulation of IZH1 and IZH2. IZH1-lacZ and IZH1_mutZRE-lacZ (A) and IZH2-lacZ and IZH2_mutZRE-lacZ (B) reporter fusion constructs show Zap1p-dependent induction during Zn²⁺-limitation. (C) A similar analysis of the IZH3-lacZ, HIS4-lacZ, and CYC1-lacZ control constructs. (D) The IZH2-lacZ reporter responds to both Zn²⁺ and exogenous myristate (C14:0). Black bars show reporter activity in – Zn²⁺, and gray bars show reporter activity in + Zn²⁺. In this and all other figures showing lacZ data, a representative experiment performed in triplicate is shown and the error bars represent ±1 SD.