Figure 3. miR-34c attenuated EMT induced by UUO via down-regulation of Jag1.

(A) Real-time PCR of miR34c, Snail1 and Vimentin in mouse kidneys with or without ureteral obstruction under administration of miR-34c or sham. (B) Western blot of Jag1 and β-actin. The bands of Jag1 were quantified using ImageJ. Control; contralateral kidneys of UUO-operated mice. Obstructed; UUO kidneys of UUO-operated mice. Sham; sham-injected mice. miR-34c; precursor of miR-34 injected mice. The original whole blot pictures are available in Supplementary Fig. S4. The original whole blot pictures of control v.s. obstructed in a same membrane are also available in Supplementary Fig. S4. (sham n = 5, miR-34c n = 4).