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. 2014 Jan 27;33(1):12. doi: 10.1186/1756-9966-33-12

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Copyright © 2014 Zhou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot analysis of TWIST expression in chemoresistant and control osteosarcoma (OS) tissues. (A) OS tissue lysates from the chemoresistant OS and the non-chemoresistant control groups (n = 6 each) were subject to western blot analysis for TWIST expression. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) blotting was used as a loading control. Lanes 1, 3, 5, 7, 9, 11 were samples from the chemoresistant OS group. Lanes 2, 4, 6, 8, 10, 12 were samples from the control group. (B) Density of the TWIST blots was normalized against that of GAPDH to obtain a relative blot density. The relative TWIST blot density of the non-chemoresistant control group was expressed as fold changes to that of the chemoresistant OS group (designated as 1). *p < 0.05 vs chemoresistant OS.