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. 2014 Jan 15;12:3. doi: 10.1186/1477-5956-12-3

Table 1.

List of proteins with different abundance in cancer cell lines

Protein name
 Ascension number (Swissprot)/matched 2D coordinates
 MW/pI
 Peptides (% cov)
 Relative change vs normal
 Zoomed in gel image showing representative spots
Abbreviation
 (World-2DPAGE portal)
    Theory Experimental     Normal Cancer
Structural
20
 Stathmin (Phosphoprotein p19)
 P16949 /SPOT 2D-001YH8
 17.3/5.76
 >15.0/5.7
 4 (18%)
 +1.6
 Inline graphic
 Inline graphic
STMN1
1
 Tropomyosin alpha-3 chain (Tropomyosin-3)
 P06753 /
 32.8/4.68
 >30.0/4.6
 6 (13%)
 -1.5
 Inline graphic
 Inline graphic
SPOT 2D-001EM1
SPOT 2D-001YBH
TPM3
 SPOT 2D-001JNO
4
 Myosin regulatory light chain-2
 P19105 /
 19.8/4.67
 >15.0/4.60
 5 (17%)
 -1.9
 Inline graphic
 Inline graphic
SPOT 2D-001JUH
ML12A
 SPOT 2D-001JUS
Enzymes
6
 Lactoylglutathione lyase (Glyoxalase I). (EC 4.4.1.5)
 Q04760 /
 20.8/5.12
 >20.0/5.00
 10 (22%)
 +7.7
 Inline graphic
 Inline graphic
LGUL
 n/a
24
 Triosephosphate isomerase (EC 5.3.1.1)
 P60174 /
 26.7/6.45
 >25.0/6.5
 5 (28%)
 +2.5
 Inline graphic
 Inline graphic
TPIS
 SPOT 2D-0003MP
18
 Nucleoside diphosphate kinase A (EC 2.7.4.6)
 P15531 /
 17.1/5.83
 >15.0/5.8
 8 (31%)
 +1.5
 Inline graphic
 Inline graphic
NDKA
 n/a
21
 Ubiquitin-conjugating enzyme E2 N (EC 6.3.2.19)
 P61088 /
 17.1/6.13
 >15.0/5.9
 6 (23%)
 +2.6
 Inline graphic
 Inline graphic
UBE2N
 n/a
16
 Gamma-glutamylcyclotransferase (EC 2.3.2.4)
 O75223 /
 21.0/5.07
 >20.0/5.0
 3 (27%)
 +2.5
 Inline graphic
 Inline graphic
GGCT
 n/a
14
 Peroxiredoxin-4 (EC 1.11.1.15)
 Q13162 /
 30.5/5.86
 >30.0/5.8
 6 (16%)
 +2.2
 Inline graphic
 Inline graphic
PRDX4
 n/a
19
 Superoxide dismutase [Cu-Zn] (EC 1.15.1.1)
 P00441 /
 15.9/5.70
 >15.0/5.70
 5 (24%)
 +1.4
 Inline graphic
 Inline graphic
SPOT 2D-001F1V
SODC
 SPOT 2D-000ZXR
17
 Glutathione S-transferase P (GST class-pi). (EC 2.5.1.18)
 P09211 /
 23.3/5.43
 >25/5.6
 8 (22%)
 +1.4
 Inline graphic
 Inline graphic
SPOT 2D-001EUM
SPOT 2D-0003Q9
GSTP1
 SPOT 2D-00085X
11
 Proteasome subunit beta type 4 precursor. (EC 3.4.25.1)
 P28070 /
 29.2/5.72
 29.0/5.7
 6 (15%)
 +1.6
 Inline graphic
 Inline graphic
SPOT 2D-000851
PSB4
 SPOT 2D-000D4M
Regulatory proteins
3
 14-3-3 protein beta/alpha
 P31946 /
 28.0/4.70
 >25.0/4.50
 6 (4%)
 +1.8
 Inline graphic
 Inline graphic
1433B
 n/a
12
 Prohibitin
 P35232 /
 29.8/5.57
 >25.0/5.60
 5 (12%)
 +1.6
 Inline graphic
 Inline graphic
PHB
 SPOT 2D-001EOL
13
 Proteasome activator complex subunit 1
 Q06323 /
 28.7/5.78
 >25.0/5.70
 2 (4%)
 -1.7
 Inline graphic
 Inline graphic
PSME1
 SPOT 2D-001YC7
16
 Proteasome activator complex subunit 2
 Q9UL46 /
 27.3/5.44
 >30.0/5.4
 4 (11%)
 -2.3
 Inline graphic
 Inline graphic
PSME2
 n/a
2
 14-3-3 protein sigma (Stratifin)
 P31947 /
 27.8/4.68
 >25.0/4.50
 9 (24%)
 -2.0
 Inline graphic
 Inline graphic
1433S
 SPOT 2D-001EP6
22
 Stress-induced-phosphoprotein 1
 P31948 /
 62.6/6.4
 >55.0/6.50
 11 (12%)
 +2.1
 Inline graphic
 Inline graphic
STIP1
 n/a
10
 Interleukin-1 receptor antagonist protein precursor
 P18510 /
 20.0/5.83
 >20.0/5.3
 2 (8%)
 +1.3
 Inline graphic
 Inline graphic
IL1RA
 n/a
5
 Rho GDP-dissociation inhibitor 1
 P52565 /
 23.2/5.02
 30.0/5.0
 4 (14%)
 +2.6
 Inline graphic
 Inline graphic
GDIR
 n/a
9
 Ran-specific GTPase-activating protein
 P43487 /
 23.3/5.19
 30/5.2
 5 (18%)
 +1.8
 Inline graphic
 Inline graphic
RANG
 n/a
Chaperones
15
 Heat-shock protein beta-1
 P04792 /
 22.8/6.00
 >20.0/5.8
 11 (32%)
 +3.1
 Inline graphic
 Inline graphic
SPOT 2D-001JQI
HSPB1
 SPOT 2D-001JQP
23
 T-complex protein 1 subunit zeta-2
 Q92526 /
 57.7/6.63
 >55.0/6.8
 2 (2%)
 -1.6
 Inline graphic
 Inline graphic
TCPW
 n/a
Others
8
 Chloride intracellular channel protein 1 (membrane associated)
 O00299 /
 26.9/5.09
 >30.0/5.1
 14 (46%)
 -1.7
 Inline graphic
 Inline graphic
  CLIC1 SPOT 2D-001YBL            

The proteins were grouped into five functional categories – structural proteins, enzymes, regulatory proteins, chaperones and others. The accession number (swiss-prot) and matched 2D coordinates, molecular weight and pI (theoretical and experimental), number of matched peptides, percentage coverage, relative change vs normal and zoomed in gel image showing representative spots are shown.