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A typical elution profile for the chromatography of miswak α-amylase on DEAE-Sepharose column (10 × 1.6 cm i.d.) previously equilibrated with 20 mM Tris-HCl buffer, pH 7.2 at a flow rate of 60 ml/h and 3 ml fractions. Absorbant at 280 nm (•___• ), units/fraction (x ___ x).
