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Gel filtration of miswak α-amylases A1 (a), A4a and A4b (b) and A5a and A5b (c) DEAE-Sepharose fractions on Sephacryl S-200 column (90 × 1.6 cm i.d.). The column was equilibrated with 20 mM Tris-HCl buffer, pH 7.2 at a flow rate of 30 ml/h and 3 ml fractions. Absorbant at 280 nm (•___• ), units/fraction (x ___ x).
