Table 1.
Purification scheme for meswak α-amylase
| Step | Total protein (mg) | Total activity (units)* | Specific activity (units/mg protein) | Fold purification | Recovery % |
|---|---|---|---|---|---|
| Crude extract |
19.77 |
4908 |
248 |
1.0 |
100 |
| Chromatography on DEAE- Sepharose |
|
|
|
|
|
| 0.0 M NaCl (A1) |
1.83 |
1805.7 |
986.72 |
3.97 |
36.8 |
| 0.05 M NaCl (A2) |
1.16 |
488.49 |
421.11 |
1.69 |
9.95 |
| 0.1 M NaCl (A3) |
1.59 |
437.96 |
275.45 |
1.11 |
8.93 |
| 0.2M NaCl (A4) |
2.48 |
647.62 |
261.14 |
1.05 |
8.92 |
| 0.3M NaCl (A5) |
2.26 |
689.75 |
305.2 |
1.23 |
14.05 |
| 0.4 M NaCl (A6) |
1.48 |
479.24 |
323.8 |
1.30 |
9.76 |
| Gel filtration on Sephacryl S-200 |
|
|
|
|
|
| α-Amylase A1 |
0.1 |
1021 |
10210 |
41 |
20.8 |
| α-Amylase A4a |
0.12 |
163.3 |
1385 |
5.4 |
3 |
| α-Amylase A4b |
0.063 |
351 |
5571 |
22 |
7 |
| α-Amylase A5a |
0.2 |
300 |
1500 |
6 |
6.1 |
| α-Amylase A5b | 0.03 | 302 | 10066 | 40 | 6 |
*One unit of enzyme activity was defined as the amount of enzyme which liberated one μmol maltose per h under standard assay conditions.