Table 2. Rate constants obtained from best-fit to six-state kinetic model.

kT2, 106 M-1·sec-1	k-D1, sec-1	k-D2, sec-1	k1, sec-1	k2, sec-1	kterm 1, sec-1	KD1, μM	KD2, μM
0.050 ± 0.01	7.5 ± 2	>25	5 ± 2	572 ± 228	1.1 ± 0.1	1	>60

A value for K_D1 of 1 μM was assumed based on solution studies (13-15). Values for k₁, k₂, and k_{term 1} were obtained from the best fit of Eq. 4 to the run-length data in Fig. 3. A value for k_-D1 was obtained from Eq. 1 and the maximum velocity. k_T2 was obtained from the best fit of Eq. 1 to velocity data in Fig. 2a. K_D2 was obtained from the best fit of Eq. 1 to velocity data in Fig. 3. The errors of the estimates were obtained based on a nonlinear regression analysis.