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. 2004 Apr 1;101(15):5553–5558. doi: 10.1073/pnas.0307575101

Fig. 2.

Fig. 2.

COMP-Ang1 injected i.v. localizes in microvascular endothelial cells of lung and intestinal villi. (A) Mice were given COMP-Ang1 (30 μg) i.v. and killed after 10 min. The indicated tissues were immunostained with PA-hAng1FD-1 Ab and counterstained with Meyer's hematoxylin. Reddish-brown indicates immunopositive signals. (B) Mice were given COMP-Ang1 (30 μg) i.v. and killed at the indicated times. Intestinal villi were stained as described in A.(C) Part of the jejunum was homogenized, 100 μg of protein homogenates was separated by SDS/PAGE, and the quantity of COMP-Ang1 was measured by immunoblotting with PA-hAng1FD-1 Ab. Results were similar from three independent experiments. Numbers and bars on the right indicate molecular sizes (kDa).