Figure 4. Knockdown of SLC7A14 in zebrafish.

(a) Injection of 1 nl of a 0.25 mM solution of slc7a14-MO dramatically reduces eye size in zebrafish. (b) At 2dpf, 4dpf and 5dpf, the eye-to-body ratio of fish injected with the slc7a14-MO was significantly smaller than that of fish injected with the control MO. N=6, Dunnett test, ***P<0.001. (c) Light-induced locomotor response with sudden light-to-dark transitions demonstrated significant visual impairment in zebrafish injected with the slc7a14-MO. N=6 in each group. (d) Dunnett tests of the light-induced locomotor responses indicate that significant differences exist between the responses of fish with small eyes and those with normal eyes during the light-ON phase. N=10, Dunnett test, **P<0.01. Bars in the graph represent standard errors of the mean (s.e.m.).