Table 1.
Purification of β-sitosterol (1) from crude soybean oil extract
|
Purification step |
Fraction |
Stigmasterol |
β-Sitosterol |
Campesterol |
|---|---|---|---|---|
| |
|
(1) |
(2) |
(3) |
| [% of total phytosterols] | ||||
| Crude soybean oil extract (S5753, Sigma-Aldrich) | ||||
| |
n.a. |
9.3 |
60.7 |
27.8 |
| Fractional crystallization | ||||
| Acetone |
S
|
8.5 |
51.2 |
40.3 |
| |
L
|
12.3 |
57.9 |
29.8 |
| Hexane/toluene/ |
S
|
12.3 |
57.7 |
30.1 |
| ethanol (4:2:1) |
L
|
4.5 |
66.0 |
29.5 |
| Diethylether |
S
|
12.4 |
61.5 |
26.2 |
| |
L
|
3.0 |
58.1 |
39.0 |
| Silica column chromatography | ||||
| |
S
|
7.1 |
78.2 |
14.7 |
| |
L
|
1.5 |
82.0 |
16.5 |
| Na-Y zeolite chromatography | ||||
| |
S
|
4.1 |
92.2 |
3.7 |
| L | 0.1 | 94.2 | 5.7 | |
Values represent averages of replicated experiments (n = 3–5).