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. 2004 Apr 5;101(15):5583–5588. doi: 10.1073/pnas.0401381101

Fig. 6.

Fig. 6.

The ectodomain of EphB4 promotes endothelial cell migration, invasion, survival, and proliferation. (Top) In a lateral migration assay, EphB4ΔC-EGFP tumor cells attract more HUVE cells (red) than EGFP-F cells. (Scale bar represents 50 μM.) HUVE cells that had migrated into the areas of green tumor cells were quantified. (Middle) Transwell or collagen-based invasion assays were used to measure HUVE cell migration or invasion toward soluble or immobilized EphB4 Fc, Fc, or basic fibroblast growth factor. (Bottom Left) HUVE cells were serum-starved in the presence of EphB4 Fc or Fc. Percentage of apoptosis was calculated by counting the number of apoptotic cells per DAPI-stained nuclei. The experiments described above were performed in triplicate and repeated at least three times. Data represent the mean ± SEM. *, P < 0.05; **, P < 0.01; and ***, P < 0.001, compared with control by one-way ANOVA and Tukey's post hoc test. (Bottom Right) HUVE cells grown in medium supplemented with EphB4 Fc or Fc were counted, and the mean ± SD is shown for one of four independent experiments. P < 0.001 by two-way ANOVA.