Gene expression profile of iVPC. RT–PCR analysis of endogenous gene expression in iVPCs and iPSCs (A) and exogenous expression of transcription factors Oct4, Klf4, Sox2, c-Myc in iVPCs (B). q RT-PCR analysis of E-cadherin (C) and cell lineage markers RUNX2 (D), GATA4 (E), and GATA6 (F) in iVPCs, ES cells and iPSCs. FACS analysis of iVPCs and iPSCs of pluripotent cell markers Oct4 and SSEA-1; hemangioblast progenitor markers CD133, Flk1, and c-kit (G).