TABLE I. Viruses analysed, polymerase chain reactions (PCRs) assays, primers' sequence, genome region and references.
| Viruses and qPCR assay | Primers and probe | Sequences 5'-3' | Genome region | References |
|---|---|---|---|---|
| RVA and PP7 multiplex RT-qPCR | NSP3 F | ACCATCTWCACRTRACCCTCTATGAGª | NSP3 | Fumian et al. (2010) |
| NSP3 R | GGTCACATAACGCCCCTATAGC | |||
| NSP3 probe | VIC-AGTTAAAAGCTAACACTGTCAAA | |||
| 247 F | GTTATGAACCAATGTGGCCGTTAT | Replicase | ||
| 320 R | CGGGATGCCTCTGAAAAAAG | |||
| 274 probe | FAM-TCGGTGGTCAACGAGGAACTGGAAC-TAMRA | |||
| HAdV qPCR | AQ1 F | GCCACGGTGGGGTTTCTAAACTT | Hexon | Heim et al. (2003) |
| AQ2 R | GCCCCAGTGGTCTTACATGCACATC | |||
| Probe | FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA | |||
| NoV RT-qPCR | COG2F | CARGARBCNATGTTYAGRTGGATGAGª | ORF1-ORF2 junction region | Kageyama et al. (2003) |
| COG2R | TCGACGCCATCTTCATTCACA | |||
| RING2-probe | FAM-TGGGAGGGCGATCGCAATCT-TAMRA | |||
| HAV RT-qPCR | Forward primer | CTGCAGGTTCAGGGTTCTTAAATC | 5' non-coding region | Villar et al. (2006) |
| Reverse primer | GAGAGCCCTGGAAGAAAGAAGA | |||
| Probe | FAM-ACTCATTTTTCACGCTTTCTG |
a: International Union of Biochemistry code (W: A/T; R: A/G; B: C/G/T; Y: C/T; N: A/C/G/T); AdV: adenoviruses; HAV: hepatitis A virus; NoV GII: norovirus genogroup II; ORF: open reading frame; qPCR: quantitative PCR; RT: reverse transcription; RVA: rotavirus species A.