TABLE III. Results of viral genome loads g-1 (dry matter) detected in anaerobically digested sludge samples using two concentration methods .
Sampling date | AdV | RVA | NoV | HAV | PP7 | |||||
---|---|---|---|---|---|---|---|---|---|---|
Method | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 |
28 Feb | 6.8 x 10 | 0.9 x 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 x 103 | ND |
02 Mar | 0 | 4.2 x 10 | 0 | 3.8 x 10 | 0 | 0 | 0 | 0 | 3.8 x 103 | 2.6 x 103 |
04 Mar | 2.1 x 10 | 6.8 x 10 | 1.6 x 102 | 0 | 0 | 0 | 0 | 0 | 2.0 x 102 | 1.4 x 102 |
14 Mar | 1.4 x 10 | 1.1 x 102 | 7.8 x 10 | 6.2 x 10 | 0 | 0 | 0 | 0 | 3.7 x 102 | 1.8 x 102 |
28 Mar | 0 | 4.3 x 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 x 108 | 3.7 x 102 |
11 Apr | 0 | 0 | 0 | 0 | 0 | 4.8 x 102a | 0 | 0 | 1.3 x 108 | 4.8 x 102 |
25 Apr | 1.0 x 10 | 2.4 x 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 x 108 | 9.4 x 107 |
16 May | 0 | 2.7 x 102 | 0 | 0 | 6.4 x 10 | 1.2 x 102 | 0 | 0 | 1.3 x 108 | 5.4 x 107 |
13 Jun | 0 | 1.9 x 102 | 0 | 0 | 1.4 x 10 | 1.5 x 102 | 0 | 2.0 x 104 | 3.1 x 107 | 5.6 x 107 |
27 Jun | 2.4 x 103 | 3.6 x 102 | 2.5 x 10a | 0 | 2.2 x 10 | 2.3 x 102 | 4.8 x 103 | 0 | ND | 9.2 x 107 |
11 Jul | 0 | 1.6 x 102 | 0 | 7.2 x 10 | 1.8 x 10 | 5.2 x 102 | 0 | 0 | 1.0 x 108 | ND |
Positive/total (n) | 5/11 | 10/11 | 3/11 | 3/11 | 4/11 | 5/11 | 1/11 | 1/11 | 10/10 | 9/9 |
a: diluted nucleic acids (1:10); AdV: adenoviruses; HAV: hepatitis A virus; Method 1: ultracentrifugation; Method 2: beef extract; ND: not done; NoV GII: norovirus genogroup II; RVA: rotavirus species.