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. 2004 Mar 19;101(15):5598–5603. doi: 10.1073/pnas.0400937101

Fig. 3.

Fig. 3.

TLR7 is required for responsiveness to VSV and influenza. (A) pDCs were purified from the bone marrow of WT, TLR7–/–, TLR9–/–, or MyD88–/– mice by flow cytometric cell sorting and infected with 5 × 106 pfu/ml VSV for 18 h. IFNα and IL-12 p40 levels were measured from culture supernatants by ELISA. (B) Total bone marrow cells were prepared from WT, TLR3–/–, TLR7–/–, and MyD88–/– mice and cultured with media or 5 × 106 pfu/ml influenza for 18 h. IFNα and IL-12 p40 levels were measured from culture supernatants by ELISA. (C) Splenocytes from WT, TLR3–/–, TLR7–/–, and MyD88–/– mice were cultured for 18 h with 1 μg/ml R848, 25 μg/ml poly(I:C), or 5 × 106 pfu/ml VSV (black lines) or medium alone (shaded area). Cells were stained and analyzed by flow cytometry. Histograms show CD69 expression levels in gated, B220+ cell populations.