Table 2.
Comparison of K. pneumoniae parent strain (KG1) and the Δ ldhA using several carbon sources in a 12-h flask cultivation, the values represent mean ± SD
| Strain | Carbon source (50 g/L) | Consumed carbon source (g/L) | 2,3-Butanediol (g/L) | L-Lactate (g/L) | D-Lactate (g/L) | Acetate (g/L) | Acetoin (g/L) | Ethanol (g/L) | Biomass (g/L) | 2,3-Butanediol yield (g/g) | R H | R C |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KG1 |
Glucose |
44.41 ± 0.45 |
13.82 ± 0.05 |
2.05 ± 0.03 |
3.34 ± 0.15 |
0.70 ± 0.04 |
0.65 ± 0.02 |
3.24 ± 0.21 |
4.63 ± 0.14 |
0.31 |
0.82 |
0.98 |
| Lactose |
24.04 ± 0.23 |
5.16 ± 0.03 |
2.05 ± 0.02 |
0.53 ± 0.03 |
1.35 ± 0.10 |
0.35 ± 0.03 |
1.29 ± 0.05 |
2.42 ± 0.08 |
0.21 |
0.66 |
1.57 |
|
| Fructose |
38.11 ± 0.32 |
13.26 ± 0.11 |
2.64 ± 0.04 |
1.53 ± 0.06 |
0.81 ± 0.06 |
0.54 ± 0.06 |
3.68 ± 0.12 |
4.02 ± 0.07 |
0.35 |
0.97 |
1.13 |
|
| Galactose |
31.52 ± 0.28 |
10.45 ± 0.08 |
1.92 ± 0.02 |
3.31 ± 0.18 |
0.80 ± 0.07 |
0.67 ± 0.04 |
3.30 ± 0.11 |
3.49 ± 0.11 |
0.33 |
1.01 |
1.28 |
|
| Maltose |
41.63 ± 0.35 |
15.05 ± 0.10 |
3.19 ± 0.03 |
2.58 ± 0.21 |
0.38 ± 0.02 |
— |
4.53 ± 0.23 |
3.54 ± 0.05 |
0.36 |
1.24 |
1.30 |
|
| ΔldhA |
Glucose |
44.74 ± 0.21 |
15.87 ± 0.08 |
2.38 ± 0.03 |
0.05 ± 0.01 |
0.83 ± 0.04 |
1.75 ± 0.10 |
5.42 ± 0.32 |
4.84 ± 0.05 |
0.35 |
1.04 |
1.13 |
| Lactose |
18.24 ± 0.15 |
2.97 ± 0.05 |
1.66 ± 0.02 |
0.44 ± 0.05 |
2.16 ± 0.04 |
0.19 ± 0.03 |
1.29 ± 0.16 |
1.57 ± 0.03 |
0.16 |
0.54 |
1.34 |
|
| Fructose |
49.52 ± 0.22 |
16.97 ± 0.24 |
3.04 ± 0.04 |
0.21 ± 0.06 |
0.30 ± 0.05 |
0.67 ± 0.02 |
4.92 ± 0.18 |
4.49 ± 0.08 |
0.34 |
1.11 |
0.99 |
|
| Galactose |
47.04 ± 0.28 |
14.33 ± 0.15 |
2.82 ± 0.02 |
0.12 ± 0.02 |
0.81 ± 0.03 |
2.11 ± 0.11 |
5.55 ± 0.22 |
4.49 ± 0.04 |
0.30 |
1.13 |
1.09 |
|
| Maltose | 49.59 ± 0.35 | 18.75 ± 0.11 | 3.95 ± 0.07 | 0.08 ± 0.02 | 0.27 ± 0.05 | 1.37 ± 0.05 | 6.01 ± 0.24 | 5.06 ± 0.15 | 0.38 | 1.18 | 1.22 |