Fig. 5.
Template-based development of second-generation MV inhibitors. (A) Structures of compounds AM-4 and AM-5. The scheme shows predicted hydrogen bonding for AM-4 in comparison with OX-1. The amide carbonyl and ortho-hydroxy groups of AM-4 engage in simultaneous hydrogen bonding with R268. (B) Connelly surface model of AM-4 docked into the F protein cavity, followed by low-temperature MD. (C) Antiviral activity of AM-4. Cells infected with MV-Edm, MV-F (V94G), or hPIV2 as indicated were incubated in the presence of different AM-4 concentrations, followed by TCID50 titration.