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Figure 2. flg22 induces RLCKs phosphorylation. (A) flg22 induces BLK phosphorylation. (B) flg22 induces PBS1 phosphorylation. (C) PBS1 phosphorylation is independent of BIK1. Protoplasts were isolated from Col-0 or bik1 mutants, and transfected with HA epitope-tagged BLK or PBS1 for 6 h and treated with 1 μM flg22 for 10 min. The CIP treatment was performed at 37 °C for 1 h. The protein was analyzed by western blot with an anti-HA antibody.
