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. 2014 Mar 22;14:73. doi: 10.1186/1471-2180-14-73

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Copyright © 2014 Zhong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sequence analysis of VirB1-89K. (A) Phylogenetic analysis of VirB1-89K. Sequence alignment and phylogenetic analysis of VirB1-89K homologs were performed using MEGA 5.1 software. Values at nodes indicate bootstrap values for 500 replicates. (B) Analysis of the tertiary structure of the CHAP domain of VirB1-89K by using the online server SWISS-MODEL. (C) Visualization of the surface active site of the CHAP domain by using PyMOLviewer, showing the cysteine residue in green and histidine in red.