Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Relevant characteristics * | Source/reference |
|---|---|---|
|
Strains |
|
|
|
S. suis
|
|
|
| 05ZYH33 |
A highly virulent strain isolated from a dead patient with STSS |
Lab collection |
| ΔvirB1-89K |
An isogenic virB1-89K mutant of strain 05ZYH33; Spcr |
[12] |
| CΔvirB1-89K |
Complemented strain of ΔvirB1-89K; Spcr; Cmr |
[12] |
|
M. lysodeikticus
|
|
|
| ATCC4698 |
Suitable for substrate for the assay of lysozyme |
Sigma-Aldrich |
|
E. coli
|
|
|
| DH5α |
Cloning host for maintaining the recombinant plasmids |
Lab collection |
| BL21(DE3) |
Expression host for exogenous protein production |
Lab collection |
|
Plasmids |
|
|
| pMD19-T |
Cloning vector; Ampr |
TaKaRa |
| pET-21a(+) |
His-tag fusion expression vector; Ampr |
Novagen |
| pET21a-CHAP | A recombinant vector with the background of pET-21a(+), designed for expression of the CHAP domain of VirB1-89K; Ampr | This work |
*Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Spcr, spectinomycin resistant.