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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: Cell Host Microbe. 2014 Feb 12;15(2):153–163. doi: 10.1016/j.chom.2014.01.008

Figure 2.

Figure 2

Accumulation of TDM in mc27000:ΔtdmhMtb. (A) Schematic representation of genomic organization of rv3451 (tdmhMtb) and rv3452 (tdmhMtb2), and the allelic exchange substrate including upstream (ups) and downstream (ds) regions used for replacement of tdmhMtb with hygr. (B). Southern blot of BsaAI digested genomic DNA from the parent wild-type (mc27000) and two hygr colonies (ΔtdmhMtb) probed with ups. (C) A representative radio-TLC of apolar lipids extractable from Mtb strains, mc27000 and mc27000:ΔtdmhMtb at 2, 3, and 4-week stages of biofilms. Purified TDM is the marker. (D) Quantitative analysis of the level of TDM as a percentage of total apolar lipids in mc27000 (wild-type) and mc27000:ΔtdmhMtb (mutant) in 2- and 4- week stages of biofilms (also see figure S2 for complete lipid profile). (Figure 2, related to Figure S2)