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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: Cell Host Microbe. 2014 Feb 12;15(2):153–163. doi: 10.1016/j.chom.2014.01.008

Figure 4.

Figure 4

Ectopic induction of TDM hydrolase increases non-selective permeability of Mtb envelope. (A) and (B), Western blot and lipid analysis of acetamide induced expression of tdmhMs in mc27000:pAO10, and the empty plasmid control, mc27000:pLAM12, in Sauton’s media for time (day) indicated on each lane. Purified TDM and FMs were markers on TLC in panel B. (C) Accumulation of glycerol, phosphorus and acetate over two hours by acetamide induced expression of tdmhMs in mc27000:ΔtdmhMtb:pAO10, and the empty plasmid control, mc27000:pLAM12, in Sauton’s media. (D) Sensitivity of the two Mtb strains described in panel E to H2O2 (40mM), lysozyme (6μg/mL) and LL37 (20μM). (E) Growth of Mtb (Erd):ΔtdmhMtb:pAO10 and Mtb(Erd):ΔtdmhMtb:pLAM12 in media with limiting glycerol (0.1% v/v) and inducer (0.2% v/v acetamide).