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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: Cell Host Microbe. 2014 Feb 12;15(2):153–163. doi: 10.1016/j.chom.2014.01.008

Figure 5.

Figure 5

The opposing effects of TdmhMtb self-restrict intracellular growth of Mtb in immunocompetent host. (A) Accumulation of radioactivity over a 48-hour period in the intracellular mc27000, mc27000: ΔtdmhMtb, and mc27000: ΔtdmhMtb:p5152 when 14C-acetate was added to the medium of infected RAW264.7 (mean ± SE, n=7). (B) Survival of Mtb (Erd), Mtb (Erd):ΔtdmhMtb and Mtb (Erd):ΔtdmhMtb:p5152 in activated BMM from C57BL/6 mice (also see Fig S4C for raw cfu). (C) Sensitivity of intracellular Mtb (Erd), Mtb (Erd):ΔtdmhMtb and Mtb (Erd):ΔtdmhMtb:p5152 to 5-day exposure of isoniazid (0.75μg/mL) added to the culture medium of infected primary BMM (C57BL/6) (mean ± SE, n=5). (D) Burden of the three Mtb strains in the lungs of C57BL/6 mice three weeks after aerosolized infection with ~50-100 bacilli/lung. Data represents two independent exposures, each with five animals/per strain (p < 0.0001, Mann-Whitney test). (E) Relative change in intracellular accumulation of radioactivity over a 48-hour period between mc27000: ΔtdmhMtb constitutively expressing tdmhMs (mc27000:ΔtdmhMtb: pYY1) and its corresponding empty vector control, mc27000: ΔtdmhMtb:pJL37, when 14C-acetate was added to the medium of infected RAW264.7 (mean ± SE, n=4). (F) Sensitivity of intracellular Mtb(Erd):ΔtdmhMtb:pYY1 and Mtb(Erd):ΔtdmhMtb:pJL37 to 5-day exposure of isoniazid (0.75μg/mL), added to the culture medium of infected primary BMM from C57BL/6 mice (mean ± SE, n=6). (G) Burden of Mtb(Erd):ΔtdmhMtb:pYY1 and Mtb(Erd):ΔtdmhMtb:pJL37 in the lung of C57BL/6 mice after three weeks of infection (p < 0.01, Mann-Whitney test). (Figure 5, related to Figure S4)