Figure 6.

Tdmh facilitates Mtb growth in MyD88^−/− mice. (A) Growth of Mtb (Erd) or Mtb (Erd):Δtdmh_Mtb and the complemented Δtdmh_Mtb:p5152 strains in activated primary BMMs from MyD88^−/− mice. (B) Accumulation of radioactivity over a 48-hour period in the intracellular mc²7000 and mc²700:Δtdmh_Mtb, when ¹⁴C-acetate was added to the medium of infected primary BMMs from MyD88^−/− mice. (C) & (D) Relative difference between the survival of Mtb (Erd):Δtdmh_Mtb expressing tdmh_Ms (Mtb(Erd): Δtdmh_Mtb pYY1) and its corresponding empty vector control, Mtb (Erd):Δtdmh_Mtb:pJL37, in primary BMMs from C57BL/6 (C), and MyD88^−/− (D) mice. (E) Burden of Mtb(Erd), Mtb(Erd):Δtdmh_Mtb and Mtb(Erd):Δtdmh_Mtb:pYY1 in the lungs of MyD88^−/− mice after 14 days of aerosolized infection (n= 5, p < 0.05, Mann-Whitney test). Mean burden of the three strains after 24 hours of exposure were 93, 89 and 99, respectively. (F) Survival of MyD88^−/− mice infected with each of the three strains described in panel E (n = 8, p < 0.001, Mann-Whitney test). Raw cfu data for panel A, C and D are provided in figures S5A S5B and S5C, respectively. (Figure 6, related to Figure S5)