Skip to main content
. 2014 Apr 3;10(4):e1004251. doi: 10.1371/journal.pgen.1004251

Figure 4. rAAV gene targeting efficiency correlates with HR, and not single strand annealing, activity.

Figure 4

(A) The HR assay. SceGFP is a full-length GFP gene disrupted by an I-SceI site. HR between SceGFP and the internal GFP (iGFP) fragment on the same plasmid upon I-SceI digestion restores GFP activity. (B) The single strand annealing assay. 5′GFP and 3′SceGFP are GFP fragments bearing 266 bp of homology. Single strand annealing repair of the I-SceI-induced DSB generates a functional GFP gene. (C) The efficiency of HR, single strand annealing and rAAV gene targeting. The indicated cell lines were analyzed using the HR and single strand annealing assays as well as for rAAV gene targeting. The mean ± SEM of three independent experiments is shown.