Skip to main content
. 2014 Apr 3;10(4):e1004252. doi: 10.1371/journal.pgen.1004252

Figure 5. TIM-CLKGR flies display quasi-normal locomotor activity rhythms despite impaired transcriptional oscillations in the brain.

Figure 5

A. Expression of CLKGR leads to impaired transcriptional oscillations in brains in culture. Luciferase recordings from cultured isolated brains of TIM-CLKGR and control flies. In both cases, we assayed females flies; n = 8 for each strain, brains were maintained at 12 hours light, 12 hours dark light regime (LD). A representative experiment (out of three) is showed. B. Addition of dexamethasone induces CLK-driven transcription in cultured TIM-CLKGR brains. Same TIM-ClKGR brains as A. Luminescence mean values after 48 hours exposure to dexamethasone (DEX) (DEX n = 8, vehicle n = 7). A representative experiment (out of three) is showed. Error bars represent SEM. Wilcoxon test was performed to determine statistical significance **p<0.01. C. TIM-CLKGR flies display less robust rhythmic behavior after long time in constant darkness. Average locomotor activity of TIM-CLKGR and control flies during five days periods in constant darkness (DD). Behavior was plotted for the first 5 days in constant darkness (DD1–5) days 6 to 10 in constant darkness (DD6–10) and days 11 to 15 in constant darkness (DD11–15). Fly strains: TIM-CLKGR, TIMGAL4 (tim-gal4/+) and UAS-CLKGR (UAS-ClkGR/+) (rhythms data and flies numbers are shown in Figure S6.) D. TIM-CLKGR flies display spread peaks of activity after prolonged times in constant darkness. We plotted the distribution of the activity peaks of individual flies in a circular chart. We did so at different times after starting the experiment: the last day of 12∶12 LD conditions, first day in constant darkness (DD1) and day 10 in constant darkness (DD10). White circles represent TIM-CLKGR individual flies. I) Black dots represent UAS-CLKGR (UAS-ClkGR/+) individual flies. II) Black dots represent TIMGAL4 (tim-gal4/+) individual flies.