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. 2014 Apr 3;10(4):e1004252. doi: 10.1371/journal.pgen.1004252

Figure 6. Coordinate amplitude between neuronal groups and the neuropeptide PDF are necessary for compensation of low amplitude molecular oscillations.

Figure 6

A. Expression of CLKGR in the pdf-expressing cells (PDF-CLKGR flies) impairs circadian locomotor behavior. Average of locomotor activity of PDF-CLKGR and control flies during five days periods in constant darkness (DD). Behavior was plotted for the first 5 days in DD (DD1–5), days 6 to 10 (DD6–10) and days 11 to 15 (DD11–15). Fly strains: PDF-CLKGR (pdf-ga4/+ UAS-ClkGR/+), PDFGAL4 (pdf-ga4/+) and UAS-CLKGR (UAS-ClkGR/+). Rhythms data and flies numbers are shown in Figure S6. Rhythmic percentages were calculated using chi square power. p<0.05. B. Han (Pdf receptor mutants) and pdf01 mutations genetically interact with expression of the ClkGR transgene. Actograms show average locomotor activity at day 2 to day 5 in constant darkness (DD2–DD5). Fly strains: TIM-CLKGR, TIMGAL4 (tim-gal4/+), UAS-CLKGR (UAS-ClkGR/+), TIM-pdf01 (tim-gal4/+;pdf01), UASCLKGR-pdf01 (UAS-ClkGR/+ pdf01), TIM-CLKGR-pdf01 (tim-gal4/+;UAS-ClkGR/+-pdf01), HAN-TIM (han3369;tim-gal4/+), HAN-CLKGR (han3369;;UAS-ClkGR/+), HAN-TIM-CLKGR (han3369;tim-gal4/+;UAS-ClkGR/+). Rhythms data and flies numbers are shown in Figure S9. Rhythmic percentages were calculated by chi square power p<0.05.