Figure 5. SEM of infected in vitro grown B. turicatae and salivary glands from infected O. turicata (A–F).

Images of in vitro cultured spirochetes (A) were used as reference for spirochete size and shape. A dorsal view of a tick in which the cuticle and midgut were removed, where white ([ ]) indicate the location of the salivary glands (B). An intact salivary gland was removed and the white and black (→) indicates an individual acinus and excretory duct, respectively (C). A cluster of cryofractured acini (D) and a magnified single acinus (E). The lumen of an ancinus was analyzed for the presence of spirochetes (F), as represented by white (→). The black boxes represent progressively magnified (D–F). The scale in nm and µm are represented by black bars.