Table 5. Genes identified as differentially expressed in both the transfected human (SK-N-SH) and mouse (Mecp2 knock-out fibroblast) cells.

	Treatment (Infection)	Different-ially Regulated Gene	Fold Change in SK-N-SH Cells by Microarray	Fold Change in Primary Mouse Fibroblasts by Microarray	Fold Change in SK-N-SH Cells by Real-Time RT-PCR	Fold Change in Primary Mouse Fibroblasts by Real-Time RT-PCR	Chahrour1 Null/WT	Chahrour1 Tg/WT
Confirmed	MECP2e1	DOCK8	3.1 (p = 0.004)	2.0 (p = 0.015)	Induced by e1%	1.3±0.5 (p = 0.269)
Inconclusive	MECP2e1	TSHR	4.1 (p = 0.042)	2.3 (p = 0.016)	*	*
	MECP2e1	BMX	3.7 (p = 0.049)	2.9 (p = 0.044)	3.4±0.9 (p = 0.333)	1.5±0.5 (p = 0.226)
	MECP2e1	KCNJ9	0.4 (p = 0.014)	0.5 (p = 0.009)	*	*
	MECP2e1	ZBTB10	0.5 (p = 0.005)	0.5 (p = 0.014)	0.9±0.6 (p = 0.811)	*
	MECP2e1	RNF2	2.6 (p = 0.004)	2.3 (p = 0.013)	1.9±0.9 (p = 0.376)	2.2±0.2 (p = 0.777)	−0.131	0.387
	MECP2e1	COQ10B	2.9 (p = 0.035)	2.4 (p = 0.038)	1.2±0.1 (p = 0.428)	1.7±0.5 (p = 0.560)
	MECP2e1	LPHN3	2.4 (p = 0.022)	2.1 (0.015)	3.5±1.3 (p = 0.946)	1.9±1.7 (p = 0.362)
Inconsistent or false calls	MECP2e1	ITGBL1	0.4 (p = 0.015)	0.3 (p = 0.021)	3.7±0.1 (p = 1.62E-06)	5.0±1.3 (p = 0.197)
	MECP2e1	GPR85	0.4 (p = 0.009)	0.3 (p = 0.015)	1.4±0.2 (p = 0.008)	3.7±1.8 (p = 0.199)	0.396	−0.250
	MECP2e2	TMC4	2.3 (p = 0.028)	2.1 (p = 0.005)	0.7±0.6 (p = 0.028)	1.4±1.0 (p = 0.490)	0.414	−0.157
	MECP2e2	SMCR8	0.4 (p = 0.008)	0.4 (p = 0.048)	1.0±0.3 (p = 0.533)	2.1±1.2 (p = 0.552)

Comparison with published data from knockout (null) and transgenic mouse gene expression microarray data (Chahrour et al, 2008 [33]) is also shown.

¹Chahrour et al, 2008 [33].

^%no expression detectable by qRT-PCR in control cells (eGFP infected), thus no fold-change calculation possible.

*too low to measure.