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. 2014 Apr 3;8(4):e2783. doi: 10.1371/journal.pntd.0002783

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© 2014 Dorňáková et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Salivary proteins of T. infestans were analyzed by 2D gel electrophoresis and 2D Western blotting in order to identify the candidate exposure marker protein of 35 kDa. The figure presents an overview of nymphal (5^th instar, A) and adult (females and males, E) salivary proteins of the Bolivian T. infestans. Developmental stage specific salivary proteins in nymphal (A) and adult (E) saliva are marked with black circles. In order to improve the protein separation, crude saliva of nymphs (B) and adults (F) were isoelectric focused in the nonlinear pH range of 3–5.6. Focused proteins were blotted onto nitrocellulose and tested for their immunogenicity using a guinea pig serum from the 5^th week of triatomine exposure. IgG (C, G) and IgM antibody reactions (D, H) with nymphal (C, D) and adult (G, H) T. infestans salivary proteins were analyzed. The candidate exposure marker antigen of 35 kDa detected by IgG and IgM antibodies is marked with a red circle.