Table 3. Variables associated with the populations used to test compliance with the “4× rule” for speciation.

Population	Nucleotide diversity (π)	θ	θ×4	Sequence divergence between clades (K)	K≥4θ?
Euparyphium/Echinoparyphium-like	0.01190	0.01160	0.0464	0.21586	Yes
E. malayanum	0.15038	0.16268	0.65072	0.08904	No
E. trivolvis-like	0.00279	0.00195	0.0078	0.18251	Yes
E. revolutum-like	0.00279	0.00195	0.0078	0.15371	Yes