Figure 3.

Analysis of cellular senescence and DNA damage in the aortas of db/db and wild-type mice. A, Representative SA-β-galactosidase staining of senescent cells (blue) in descending thoracic aortas from male and female db/db and wild-type mice. B, Telomerase activity in aortas from male and female db/db and wild-type mice. C, Representative γH2AX immunostaining (green) of aortic cross-sections from male and female db/db and wild-type mice. L, lumen. Arrows indicate positive staining. D, The percentage of γH2AX-positive nuclei was compared among groups (n = 8 per group).