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. 2014 Apr 3;9(4):e93583. doi: 10.1371/journal.pone.0093583

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© 2014 Ren et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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gMDSCs and gMDSCs–pDsRed2-1 began to fuse after 2 d and myotube cells increased markedly. gADSCs and gADSCs–pDsRed2-1 began to integrate 5 d after myogenic induction, leading to short myotube cell formation. DAPI staining revealed the presence of multiple nuclei in the same myotube, and immunohistochemical staining demonstrated that the cells were fast muscle myosin (green) positive; control (uninduced) cell were fast muscle myosin negative. gADSCs, goat adipose-derived mesenchymal stem cells; gMDSCs, goat muscle-derived satellite cells.