Table 3. Maturation and development of cloned embryos derived from gADSCsa–pDsRed2-1, gMDSCsb–pDsRed2-1 and gFFCsc–pDsRed2-1 in vitro.
Group | Number of cumulus–oocyte complexes | Number of mature embryos | Number of surviving embryos | Number of embryos exhibiting convergence | Number of embryos exhibiting cleavage | Number of eight-cell embryos | Number of blastocysts | |
gADSCs–pDsRed2-1 | (Maturity rate) | (Survival rate) | (Convergence rate) | (Cleavage rate) | (Eight-cell rate) | (Blastocyst rate) | ||
A | 237 | 185 | 167 | 145 | 131 | 43 | 15 | |
(78.06%) | (90.27%) | (86.82%) | (90.34%) | (29.66%) | (10.34%) | |||
B | 281 | 205 | 188 | 152 | 130 | 41 | 15 | |
(72.95%) | (91.70%) | (80.85%) | (85.52%) | (26.97%) | (9.86%) | |||
C | 263 | 210 | 196 | 170 | 149 | 48 | 19 | |
(79.85%) | (93.33%) | (86.73%) | (87.65%) | (28.24%) | (11.18%) | |||
Total | 781 | 600 | 551 | 467 | 410 | 132 | 49 | |
(76.82%) | (91.83) | (84.75) | (87.79%) | (28.27%) | (10.49%) | |||
Sdd | 3.58 | 1.53 | 3.42 | 2.42 | 1.35 | 0.67 | ||
gMDSCs–pDsRed2-1 | A | 231 | 172 | 162 | 155 | 133 | 38 | 18 |
(74.46%) | (94.18%) | (90.12%) | (85.81%) | (24.51%) | (11.61%) | |||
B | 281 | 203 | 188 | 171 | 149 | 49 | 16 | |
(72.24%) | (92.61%) | (90.96%) | (87.13%) | (28.66%) | (9.37%) | |||
C | 161 | 122 | 110 | 101 | 83 | 27 | 9 | |
(75.78%) | (90.16%) | (91.82%) | (82.18%) | (26.73%) | (8.91%) | |||
Total | 673 | 497 | 460 | 427 | 365 | 114 | 43 | |
(73.85%) | (92.56%) | (92.83%) | (85.48%) | (26.70%) | (10.07%) | |||
Sdd | 1.79 | 2.03 | 0.85 | 2.56 | 2.08 | 1.44 | ||
gFFCs–pDsRed2-1 | A | 94 | 68 | 61 | 45 | 41 | 13 | 6 |
(72.34%) | (89.71%) | (73.77%) | (91.11%) | (28.88%) | (13.33%) | |||
B | 87 | 71 | 68 | 60 | 54 | 19 | 7 | |
(81.61%) | (95.77%) | (88.24%) | (90.00%) | (31.67%) | (11.67%) | |||
C | 120 | 88 | 79 | 56 | 49 | 17 | 5 | |
(73.33%) | (89.77%) | (70.89%) | (87.50%) | (30.36%) | (8.93%) | |||
Total | 301 | 227 | 208 | 161 | 144 | 49 | 18 | |
(75.42%) | (91.63%) | (77.40%) | (89.44%) | (30.43%) | (11.18%) | |||
Sdd | 5.09 | 3.48 | 9.30 | 1.85 | 1.40 | 2.22 |
Goat adipose-derived mesenchymal stem cells.
Goat muscle-derived satellite cells.
Goat fetal fibroblast cells.
Standard deviation.