Table 2. qPCR primer information and predicted product size.

Target bacterial group	Primers	DNA Sequence (5′- 3′)	Predicted PCR productsize (bp)	Annealingtemp (°C)	Reference
Atopobium cluster	Atopo-F	GGGTTGAGAGACCGACC	190	55	[38]
	Atopo-R	CGGRGCTTCTTCTGCAGG
Bacteroides fragilis group	Bfra-F	ATAGCCTTTCGAAAGRAAGAT	495	50	[39]
	Bfra-R	CCAGTATCAACTGCAATTTTA
Bifidobacterium genus	Bifid-F	CTCCTGGAAACGGGTGG	550	55	[39]
	Bifid-R	GGTGTTCTTCCCGATATCTACA
Clostridium coccoides group	Ccoc-F	AAATGACGGTACCTGACTAA	440	50	[39]
	Ccoc-R	CTTTGAGTTTCATTCTTGCGAA
Clostridium leptum group	sg-Clept-F	GCACAAGCAGTGGAGT	239	55	[38]
	sg-Clept-R3	CTTCCTCCGTTTTGTCAA
Clostridium perfringens group	ClPER-F	AGATGGCATCATCATTCAAC	170	60	[40]
	ClPER-R	GCAAGGGATGTCAAGTGT
Desulfovibrio genus	Dsv-F	CCGTAGATATCTGGAGGAACATCAG	135	63	[41]
	Dsv-R	ACATCTAGCATCCATCGTTTACAGC
Enterobacteriaceae family	Enter-F	TGCCGTAACTTCGGGAGAAGGCA	428	60	[42]
	Enter-R	TCAAGGACCAGTGTTCAGTGTC
Lactobacillus genus	Lac-F	AGCAGTAGGGAATCTTCCA	341	58	[43]
	Lac-R	CACCGCTACACATGGAG
Prevotella genus	g-Prevo-F	CACRGTAAACGATGGATGCC	528	55	[38]
	g-Prevo-R	GGTCGGGTTGCAGACC
All bacteria	Uni331F	TCCTACGGGAGGCAGCAGT	466	58	[44]
	Uni797R	GGACTACCAGGGTATCTATCCTGTT