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. 2014 Apr 3;9(4):e93797. doi: 10.1371/journal.pone.0093797

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© 2014 Harding et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C, CHO cells were transfected with HA-ASIC2a and/or PSD-95, with DsRed added to keep total cDNA constant. A, Coimmunoprecipitation of ASIC2a with PSD-95. In the top panels, cell lysates were immunoprecipitated (IP) with anti-PSD-95 antibody, and then immunoblotted (IB) for ASIC2a (anti-HA). The bottom panels shows immunoblots of cell lysates. B, Immunoblot (anti-HA) of biotinylated (surface) and total ASIC2a. Lanes for surface and total protein in A and B were from the same gel and film exposure respectively, but were arranged for clarity. C, Quantification of relative ASIC2a surface expression with and without coexpressed PSD-95 (n = 9; *P<0.05).