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. 2014 Apr 3;9(4):e93819. doi: 10.1371/journal.pone.0093819

Figure 5. IC50 values of excipients for CES1A1 and CES2.

Figure 5

IC50 values of SLS (A), RH40 (B), Tween 20 (C) and EL 35 (D) on the p-nitrophenyl acetate hydrolase activities of recombinant CES1A1 and CES2. Microsomes were incubated with 150 μM p-nitrophenyl acetate in the presence of excipients of various concentrations. Activities were expressed as a percentage of the tested activity compared with the control. Each data point represents the mean ± SD. The control activities by recombinant CES1A1 and CES2 were 3.95 nmol/min/mg and 40.6 pmol/min/mg, respectively.