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Figure 1. pp32 proteins and sphingosine metabolites. (A) 2D chemical structures of various sphingosine metabolites and the synthetic analog FTY720. (B) Molecular weight marker (lanes 1 and 6), pp32 full-length 1–249 (lanes 2 and 7), pp32ΔCT 1–149 (lanes 3 and 8), pp32r1 (lanes 4 and 9), pp32r1Y140H (lanes 5 and 10). Input protein is shown on the left and protein bound to biotin-FTY720 coupled streptavidin beads after washing is shown on the right.
