Figure 3. Reduced FTY720 mediated apoptosis in cells overexpressing pp32r1 or pp32r1Y140H. (A) Cells were either untreated, treated with DMSO control, or treated with 6.5 µM FTY720 and stained using an annexinV-FITC/ PI kit. Viable cells (unstained, bottom left quadrant Q4), early apoptotic cells with annexin V-FITC staining (bottom right quadrant Q3), late apoptotic cells with annexin V-FITC/PI staining (top right quadrant Q2), dead cells with PI staining (top left quadrant Q1). (B) The results from (A) for cells treated with 6.5 µM FTY720 are shown graphically as a percentage of viable Q4, apoptotic (early and late Q2/Q3) and dead cells Q1.