Skip to main content
. 2014 Apr 3;10(4):e1004040. doi: 10.1371/journal.ppat.1004040

Figure 6. Knockdown of HBZ expression or strong inhibition of NF-κB reactivates latent HTLV-1.

Figure 6

(A) Knockdown of HBZ mRNA expression in latently infected cells (LICs). LIC clones were transduced with a lentiviral HBZ shRNA construct (open bars) or with a control vector (solid bars) as previously described [30]. Transduced cells were selected in liquid medium containing 1 µg/ml puromycin. Knockdown of HBZ expression was confirmed by real-time PCR as detailed in Materials and Methods . (B) Induction of p24 expression in latently infected cell (LIC) clones after HBZ knockdown. LIC clones 1–3 and their derivatives stably transduced with lentiviral HBZ shRNA were analyzed by immunoblotting for p24, Tax, Rex, and β-actin (Actin). (C) Strong inhibition of NF-κB reactivates HTLV-1 from latently infected cells. LIC clones were transduced with a lentiviral construct, LV-ΔN-IκBα, that expresses a degradation resistant super-repressor form of IκBα, ΔN-IκBα. Transduced cells were selected as in (A) and analyzed by immunoblotting for the indicated proteins.