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. 2014 Apr 3;9(4):e93933. doi: 10.1371/journal.pone.0093933

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© 2014 Holmes et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Bisulfite-specific and methylation-specific quantitative real-time PCR analysis of mixtures of bisulfite-converted artificially methylated DNA and unmethylated DNA from sperm. The ACTB/SHOX2/SEPT9 assay amplifies bisulfite-converted methylated SHOX2 and SEPT9 gene copies and total bisulfite-converted DNA using an ACTB amplicon. Shown are mean values (± standard deviation) of triplicate measurements.