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. 2014 Apr 3;9(4):e93954. doi: 10.1371/journal.pone.0093954

Figure 1. AEA ameliorates mBSA-Induced delayed hypersensitivity.

Figure 1

Mice were sensitized to mBSA by giving an injection of 150 μg of mBSA emulsified into CFA into each hind flank. They were treated with AEA or vehicle on days 5 and 6 after sensitization and rechallenged with 200 μg mBSA into the right footpad and PBS in the left footpad. [A] The footpad swelling was measured as described in Methods. Graph representative of 3 independent experiments with n = 5. *p<0.05 compared to Veh. [B] Cells were harvested from draining lymph nodes [LN] and viable cells counted using trypan blue exclusion. Cell numbers are representative of 2 independent experiments with n = 5. #p<0.05 compared to naïve. **p<0.05 when compared to vehicle. [C] Cells from the LN were cultured for 4 hours in the presence of PMA, Ionomycin, and Golgi Plug and stained for IL-17 secreting cells. Graph is representative of 3 independent experiments with n = 3. # p<0.05 compared to naïve; *p<0.05 compared to vehicle.