Figure 5. Effect of AEA on miRNA induction in mice with mBSA-induced DTH: Affymetrix miRNA array analysis.

[A] Heat map of miRNA expression patterns. RNA was isolated from five pooled popliteal lymph nodes of mBSA-sensitized mice rechallenged with mBSA and treated with either AEA or Vehicle. The samples were analyzed using Affymetrix miRNA 1.0 microarray. The values for each sample were generated by normalizing the expression of the microRNA to each sample. Ward's Method was used for clustering patterns and the samples were ordered by the Input Rank method. [B] AEA induced differential regulation of several miRNA species, as enumerated by the Venn diagram. [C] The most differential regulated miRNA expressed as a bar chart. [D] Ingenuity Pathway Analysis of the most differentially regulated biological networks.