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. 2014 Apr 3;10(4):e1004056. doi: 10.1371/journal.ppat.1004056

Figure 1. Diagram of the lp28-1 plasmids for the wild-type, Vmp− mutant and reconstituted Vmp+R strains.

Figure 1

Schematic of a portion of the B. hermsii linear plasmid lp28-1 contained in the wild-type (WT), Vmp-minus mutant (Vmp−), and reconstituted (Vmp+R) strains. A region of the lp28-1 plasmid including the 5′ end of vlp7, Upstream Homology Sequence (UHS), promoter (P) and ribosome binding site (rbs), T-rich region, and proximal inverted repeat was replaced with the flgBp-kan cassette to construct the Vmp− strain. DHS = Downstream Homology Sequence. Arrowheads with numbers indicate primer number and location. Arrows oriented toward each other indicate inverted repeats that coincide with the stems in the potential stem loop structures. ORFs in grey are plasmid replication genes. Light-shaded ORFs indicate vmp silent cassettes. Dark-shaded ORF is the vmp in the expression site (vlp7). Restriction sites for Southern blot analysis are indicated on the Vmp− plasmid. The reconstituted strain Vmp+R has the wild-type lp28-1 containing the flaBp-aacC1 cassette.