Figure 2. GM-CSF promotes development of CD103⁺ DCs and expression of CD103 in young and adult mice, respectively.

Analysis of lung DC subsets in (A–C) 6–8 week-old and (D–E) 6-day-old mice. (A, D) Gated cells in dot plots show CD103⁺ and CD11b⁺ DCs with values indicating percentages among CD11c⁺Siglec-F⁻ lung DCs. Cells were pre-gated on CD45⁺eFluor780⁻ viable cells. (B,E) Bar graphs depict total number of CD103⁺ and CD11b⁺ DCs in the lung. (C) Mean fluorescence intensity (MFI) of CD103 expression on CD103⁺CD11b⁻ DCs. Values shown represent the mean ± SD (n = 3–4). (F) Analysis of lung DC subsets in mixed BM chimeras generated by transfer of 1∶1 mixture of CD45.1⁺WT∶CD45.2⁺ Csf2rb ^−/− or control CD45.1⁺WT∶CD45.2⁺WT into lethally irradiated recipients. Bar graphs show the percentage of CD45.2⁺WT and CD45.2⁺ Csf2rb ^−/− cells among CD103⁺ and CD11b⁺ DCs in the lung. The mean ± SD is shown (n = 3).